Tabula Muris

Tabula Muris is a compendium of single cell transcriptome data from the model organism Mus musculus, containing nearly 100,000 cells from 20 organs and tissues. The data allow for direct and controlled comparison of gene expression in cell types shared between tissues, such as immune cells from distinct anatomical locations. They also allow for a comparison of two distinct technical approaches:

• microfluidic droplet-based 3’-end counting: provides a survey of thousands of cells per organ at relatively low coverage
• FACS-based full length transcript analysis: provides higher sensitivity and coverage.

We hope this rich collection of annotated cells will be a useful resource for:

• Defining gene expression in previously poorly-characterized cell populations.
• Validating findings in future targeted single-cell studies.
• Developing of methods for integrating datasets (eg between the FACS and droplet experiments), characterizing batch effects, and quantifying the variation of gene expression in a many cell types between organs and animals.

The peer reviewed article describing the analysis and findings is available on Nature.