Data

Data for this project are accessible through Figshare. These include:

  • Gene count tables derived from SMART-Seq2 RNAseq libraries prepared from individually FACS sorted cells. This includes 53,760 cells from 20 tissues from 8 mice.
  • Gene count files derived from cells prepared using the 10x Genomics platform and processed with CellRanger. This contains sequence data collected from 422,803 droplet libraries, 55,656 of which passed a QC cutoff of 500 genes and 1000 UMI.
  • Metadata for single cells (mouse id, sex, FACS setting, etc), along with annotations for the cells.
  • Robject files for easy loading with the Seurat package, including annotations and clustering for cells from each tissue, prepared from microfluidic droplet-based sequencing libraries or libraries from FACS-sorted cells.
  • tSNE plots with the expression of the top 10 genes for each tissue projected onto the plot.